Functional Testing of a Bicistronic Retroviral Vector for Intracellular Peptide Production
نویسندگان
چکیده
منابع مشابه
Functional testing of a bicistronic retroviral vector for intracellular peptide production.
Cytotoxic T cells generally recognize short 8-mer to 11-mer peptides in association with major histocompatibility complex (MHC) class I molecules on the surface of the antigen presenting cells (APCs). Usually, these peptides are produced in the cytosol of the APCs by proteolytic degradation of cytosolic proteins. The peptides are subsequently transported first to the endoplasmatic reticulum (ER...
متن کاملProduction of human factor VIII-FL in 293T cells using the bicistronic MGMT(P140K)-retroviral vector.
Hemophilia A is the most common X-linked bleeding disorder; it is caused by deficiency of coagulation factor VIII (FVIII). Replacement therapy with rFVIII produced from human cell line is a major goal for treating hemophilia patients. We prepared a full-length recombinant FVIII (FVIII-FL), using the pMFG-P140K retroviral vector. The IRES DNA fragment was cloned upstream to the P140K gene, ...
متن کاملDevelopment of a new bicistronic retroviral vector with strong IRES activity
BACKGROUND Internal Ribosome Entry Site (IRES)-based bicistronic vectors are important tools in today's cell biology. Among applications, the expression of two proteins under the control of a unique promoter permits the monitoring of expression of a protein whose biological function is being investigated through the observation of an easily detectable tracer, such as Green Fluorescent Protein (...
متن کاملfaciliting lexical access for the fluent production of speech
the hypothesis is that recent and frequent exposure to lexical items leads to a more fluent production of speech in terms of rate of speech. to test the hypothesis,a one-way anova experimental design was carried out. 24 sednior students of efl participated in a one-way interview test. data analyses revealed that those who were exposed frequently to the lexical items over a week prior to inter...
15 صفحه اولfacilitating lexical access for the fluent production of speech
the hypothesis is that recent and frequent exposure to lexical items leads to a more fluent production of speech in terms of rate of speech. to test the hypothesis , a one- way anova experimental design was carried out. 24 senior student of efl participated in a one-way interview test. data analyses revealed that those who were exposed frequently to the lexical items over a week prior to inte...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99266bm04